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. 2019 Oct 3;12:8125–8138. doi: 10.2147/OTT.S224721

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© 2019 Chen et al.

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Snail is involved in CXCL8-regulated glioma cells proliferation, migration and invasion. (A) Correlation analysis of CXCL8 and Snail mRNA expression by qRT-PCR. (B, C) The over-expression efficiency against Snail was verified by qRT-PCR and Western blotting in U-251MG and U-87MG cells. (D) Growth curves between None, Vector, CXCL8 (KD), Snail (OE) and CXCL8 (KD) + Snail (OE) by CCK-8 assay. The results are shown as the Mean ± Standard Deviation (SD) of three independent experiments. (E–H) The histogram quantification of the EdU (E), colony formation assay (F), Transwell migration (G) and invasion assays (H) with U-251MG and U-87MG cells. All the results indicated that Snail over-expression abrogated the effects of CXCL8 knockdown on inhibiting U-251MG and U-87MG cell proliferation (D, E), colony formation (F), migration (G) and invasion (H). Statistical significance was assessed using two-tailed Student’s t test (A and B) and one-way ANOVA followed by Dunnett’s tests for multiple comparisons (D-H). *p < 0.05, **p < 0.01 and ***p < 0.001.