Fig. 3.

LHA-projecting AgRP neurons do not affect sweet taste sensitivity after CTA. a Schematic image of the injection of AAV-DIO-ChR2-EYFP into the ARC with bilateral optic fiber insertion above the LHA for photostimulation in the AgRP-ChR2-EYFP mouse. b Representative image showing ChR2-EYFP expression at axon terminals of AgRP neurons in the LHA and the approximate placement of an optic fiber (dashed lines). c Licks for 300 mM sucrose in 10 s before and after CTA conditioning. n = 6, paired Student’s t test. d Brief access taste tests for sucrose solution in the presence or absence of optogenetic activation of LHA-projecting AgRP neurons after CTA conditioning. n = 6, F = 1.272, and P = 0.262, two-way ANOVA with Bonferroni post hoc test. All experiments were carried out with 10- to 16-week-old male mice. Data are given as means ± SEM. ***P < 0.001