Table 1.
Comparison of genome assembly and completeness assessment results between the previous genome (Tuna_128) and new genome assembled in this study (Tuna_2) using CEGMA and BUSCO.
| Assembly Statistics | Tuna_1 | Tuna_2 |
|---|---|---|
| Contig statistics | ||
| Number of contigs | 135,841 | 1,248 |
| Total contig size (bp) | 684,478,122 | 786,014,188 |
| Contig N50 size (bp) | 8,173 | 3,075,225 |
| Largest contig (bp) | 79,059 | 14,489,242 |
| Scaffold statistics | ||
| Number of scaffolds | 16,801 | 444 |
| Total scaffold size (bp) | 740,348,846 | 786,596,543 |
| Scaffold N50 size (bp) | 136,950 | 7,922,002 |
| Largest scaffold size (bp) | 1,021,118 | 19,788,065 |
| GC content (%) | 40 | 40 |
| Number of gaps | 119,041 | 804 |
| Completeness Assessment Results Using CEGMA | ||
| Total number of core genes queried | 233 | 233 |
| Number of core genes detected | ||
| Complete | 176 (75.54%) | 222 (95.28%) |
| Complete + Partial | 222 (95.28%) | 225 (96.57%) |
| Number of missing core genes | 11 (4.72%) | 8 (3.43%) |
| Average number of orthologs per core genes | 1.38 | 1.03 |
| % of detected core genes that have more than 1 ortholog | 26.14 | 3.15 |
| Completeness Assessment Results using BUSCO | ||
| Total BUSCO groups searched | 4584 | 4584 |
| Complete BUSCOs | 4021 (87.7%) | 4044 (88.2%) |
| Complete and single-copy BUSCOs | 3933 (85.8%) | 3848 (83.9%) |
| Complete and duplicated BUSCOs | 88 (1.9%) | 196 (4.3%) |
| Fragmented BUSCOs | 337 (7.4%) | 222 (4.8%) |
| Missing BUSCOs | 226 (4.9%) | 318 (7.0%) |