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. 2019 Oct 8;9:14432. doi: 10.1038/s41598-019-51016-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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cAMP antagonism by ECD-scFvhFc. (A) A schematic representation outlining the effect of ECD-scFvhFc on the EC₅₀ of PTH (1–34) mediated cAMP production. (B) A representative plot showing the effect of ECD-scFvhFc on the PTH (1–34) concentration-response curves. PTH1R expressing CHOK-1 cells were incubated with different concentrations of ECD-scFvhFc (indicated by the different coloured traces) followed by incubation with a concentration-range of PTH (1–34). The cAMP concentration was calculated using cAMP standard curve and each data point represents mean and standard deviation from quadruplicate measurements. (C) Plot summarising the calculated EC₅₀ from four separate experiments. Bars represent means ± SEM.