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. 2019 Oct 8;10:4561. doi: 10.1038/s41467-019-12535-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Wide-field FLIM of Alexa Fluor 532 molecules. a Gated channel intensity. b Ungated channel intensity (scale bar 1 μm). c Measured lifetimes are plotted along with total brightness for the numbered, diffraction-limited regions with SE error bars indicated. The majority of these spots are single-molecule emitters as demonstrated by their photobleaching and blinking dynamics (Supplementary Figs. 4 and 5). Source data are provided as a source data file