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. 2019 Oct 2;10:1140. doi: 10.3389/fphar.2019.01140

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This work is authored by Jie Dong and Qiang Ma on behalf of the U.S. Government and, as regards Dr. Dong, Dr. Ma, and the U.S. Government, is not subject to copyright protection in the United States. Foreign and other copyrights may apply

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Elevated expression of TIMP1 in fibroblastic cells by MWCNTs in the lung. (A) TIMP1 in fibroblasts examined by double immunofluorescence staining of TIMP1 (green) and Hsp47 (red), with DAPI nuclear staining (blue). The full images with scale bar are shown in Figure S5 . (B) TIMP1 in myofibroblasts examined by double immunofluorescence staining of TIMP1 (green) and α-SMA (red), with DAPI nuclear staining (blue). The full images with scale bar are shown in Figure S6 . In (A) and (B), yellow color is generated from the overlap of green and red. (C) Number of TIMP1+ Hsp47+ cells per unit area (2x10⁴ µm²) is presented as mean ± SD (n = 4). (D) Number of TIMP1+ α-SMA+ cells per unit area (2x10⁴ µm²) is presented as mean ± SD (n = 4).