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. 2019 Oct 2;10:2324. doi: 10.3389/fimmu.2019.02324

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Copyright © 2019 Wu, Fu, Yin, Guo, Wang and Ye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Anti-TNP antibody titers and affinities induced by trinitrophenylated-keyhole limpet hemocyanin (TNP-KLH) (n = 5). The dynamics of anti-TNP antibody titers were detected by standard antibody titration ELISA (A). The T1/T8 ratio was used to present the dynamic of antibody maturation (B), and affinity partitioning ELISA (C) was used to detect the differential dynamics of antibody subpopulations at weeks 2, 6, and 18 post-immunization. Week 0 was bled prior to immunization, and PBS was immunized as the control. Data represent the mean ± standard deviation (SD) of five individual fish at each time point and are representative of three independent experiments.