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. 2019 Oct 2;10:2324. doi: 10.3389/fimmu.2019.02324

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Copyright © 2019 Wu, Fu, Yin, Guo, Wang and Ye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ELISPOTs of anti-TNP-specific antibody-secreting cells (ASCs) (in vivo) in immune tissues. The ELISPOTs of the in vivo ASC response within peripheral blood (PBL), spleen (SPL), and anterior kidney (AK) were shown from an individual fish immunized with TNP-KLH by 1 × 10⁶ leukocytes per well (A). The average number of ASCs per 10⁶ leukocytes (B,D) and the total number of ASCs in each tissue (C,E) were detected at weeks 0, 2, 4, 6, 8, 10, 14, and 18 post-immunization (n = 5) with TNP-KLH (B,C) and PBS (D,E). Data represent the mean ± SD of five individual fish at each time point and are representative of three independent experiments.