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LLPCs (in vitro D15) have a strong capability to secrete antibodies compared to that of other ASCs (ex vivo and in vitro D7). ELISPOTs of TNP-specific ASCs from different immune tissues of vaccinated channel catfish 8 weeks post-immunization (2 × 106 leukocytes per well) (A). The average ELISPOT size of AK LLPCs relative to ASCs (ex vivo). Statistical difference was evaluated by t-test, and ***p < 0.001 (B).
