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. 2019 Oct 2;13:455. doi: 10.3389/fncel.2019.00455

FIGURE 12.

FIGURE 12

Representative images and quantification of the c-Fos+ neurons in the ventromedial hypothalamic nucleus (VmH). (A–J) Images and their insets of c-Fos+ cells in the VmH of male or female mice after exposure to 0.01% DCM-containing mineral oil (control), L- and H-16OH, and L- and H-16Ac. Scale bar, 200 μm. (K) L-16OH, H-16OH, L-16Ac, or H-16Ac generated more c-Fos signals than the control on both male and female VmH; H-16OH was more effective than L-16OH on both male and female VmH whereas H-16Ac did not elicit more c-Fos signals than L-16Ac on either male or female VmH (M-L-16OH vs. M-control, t = 2.965, p = 0.000; M-H-16OH vs. M-control, t = 6.602, p = 0.000; M-L-16Ac vs. M-control, t = 4.892, p = 0.000; M-H-16Ac vs. M-control, t = 7.509, p = 0.000; F-L-16OH vs. F-control, t = 3.082, p = 0.032; F-H-16OH vs. F-control, t = 15.797, p = 0.000; F-L-16Ac vs. F-control, t = 3.243, p = 0.021; F-H-16Ac vs. F-control, t = 3.435, p = 0.012; M-H-16OH vs. M-L-16OH; t = 3.097, p = 0.032; F-H-16OH vs. F-L-16OH, t = 12.715, p = 0.000; M-H-16Ac vs. M-L-16Ac, t = 2.617, p = 0.117; F-H-16Ac vs. F-L-16Ac, t = 0.192, p = 1.000). (L,M) The female VmH was more responsive than the male VmH to H-16OH whereas the opposite was true to H-16Ac. H-16OH was more effective than H-16Ac on the female VmH (F-H-16OH vs. M-H-16OH, t = 9.543, p = 0.000; M-H-16Ac vs. F-H-16Ac, t = 4.266, p = 0.000; F-L-16OH vs. M-L-16OH, t = 0.0751, p = 0.940; F-L-16Ac vs. M-L-16Ac, t = 1.841, p = 0.072; F-H-16OH vs. F-H-16Ac, t = 12.362, p = 0.000; M-H-16OH vs. M-H-16Ac, t = 1.447, p = 1.000; F-L-16OH vs. F-L-16Ac, t = 0.161, p = 1.000; M-L-16OH vs. M-L-16Ac, t = 1.927, p = 0.596). N = 6 for each group. All values are expressed as mean ± s.e.m. ∗p < 0.05, ∗∗∗p < 0.001.