TABLE 1.

Antibodies used in Western blotting (WB) or immunohistochemistry (IH) to label the NVU.

NVU Protein	Antibody	Dilution	Source
Endothelial cell	Anti-CD31	1:100	BD Biosciences, Wokingham, United Kingdom
Tight junction	Anti- zona occludens-1 (ZO-1) Anti-claudin 5	1:300 1:500	Abcam, Cambridge, United Kingdom Fisher Scientific, Loughborough, United Kingdom
Pericyte	Anti-platelet-derived growth factor receptor-β (PDGFRβ)	1:200	R&D Systems, Abingdon, United Kingdom
Smooth muscle cell	Anti-α smooth muscle actin (α-SMA) Anti-α-SMA-FITC	1:350 (WB) 1:350 (IH)	Sigma-Aldrich, Poole, United Kingdom Sigma-Aldrich, Poole, United Kingdom
Basement membrane	Anti-laminin	1:500 (WB) 1:350 (IH)	Sigma-Aldrich, Poole, United Kingdom
Astrocyte	Anti-glial fibrillary acidic protein (GFAP)	1:5000 (WB) 1:1000 (IH)	Agilent Technologies, Stockport, United Kingdom Abcam, Cambridge, United Kingdom