Figure 1:
Simultaneous detection and quantification of photoproducts and oxidative DNA damage in HEK cells exposed to UVC radiation. (a) Fluorescence image of DNA molecules labelled with the multi-labelling reaction. Grey colour represents the backbone of the DNA molecules, red dots represent photoproduct sites, and blue dots represent oxidative damage sites. (b) Analysis results of the raw image using automatic software. Green – detected DNA molecules, red dots – detected photoproduct labels, blue dots –detected oxidative damage sites. (c) Dose response of HEK cells exposed to UVC radiation as detected by the multi-labelling reaction. Red represents the levels of detected photoproducts and blue the levels of oxidative damage.