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. 2019 Oct 2;7:202. doi: 10.3389/fcell.2019.00202

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Copyright © 2019 Zhang, Li, Zou, Guo, Zhang, Xia, Zhang, Yang and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of high-concentration NEFAs and siOrai1 on Orai1 and NFκB p65 localization in BRL-3A cells. Original immunofluorescence images demonstrating nuclear staining (blue; left images), NFκB p65 (red; second images), Orai1-specific antibody (Green; third images), and an overlaying of all nuclear staining NFκB p65, and Orai1-specific antibody in BRL-3A cells incubated without (upper images) or with (second images) NEFAs (1.2 mM, 3 h), siOrai1 (third images), or siOrai1 + NEFAs (lower images). Scale bar: 50 μm.