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. 2019 Oct 2;7:202. doi: 10.3389/fcell.2019.00202

FIGURE 7.

FIGURE 7

Effect of NFκB inhibitor wogonin on ER stress in BRL-3A cells. (A) Original Western blots showing the protein expression of PERK, IRE1, ATF6, CHOP, GRP78, ORAI1, NFκB p65, and β-actin in BRL-3A cells incubated without or with NEFA (1.2 mM, 12 h) in the absence or presence of NFκB inhibitor wogonin (100 μM). (B) Arithmetic means ± SEM (n = 9) of the PERK/β-actin ratio in BRL-3A cells incubated without (white bar) or with (black bar) NEFA (1.2 mM, 12 h) in the absence (left bars) or presence (right bars) of NFκB inhibitor wogonin (100 μM). (C) Arithmetic means ± SEM (n = 9) of the IRE1/β-actin ratio in BRL-3A cells incubated without (white bar) or with (black bar) NEFA (1.2 mM, 12 h) in the absence (left bars) or presence (right bars) of NFκB inhibitor wogonin (100 μM). (D) Arithmetic means ± SEM (n = 9) of the ATF6/β-actin ratio in BRL-3A cells incubated without (white bar) or with (black bar) NEFA (1.2 mM, 12 h) in the absence (left bars) or presence (right bars) of NFκB inhibitor wogonin (100 μM). (E) Arithmetic means ± SEM (n = 9) of the GRP78/β-actin ratio in BRL-3A cells incubated without (white bar) or with (black bar) NEFA (1.2 mM, 12 h) in the absence (left bars) or presence (right bars) of NFκB inhibitor wogonin (100 μM). (F) Arithmetic means ± SEM (n = 9) of the ORAI1/β-actin ratio in BRL-3A cells incubated without (white bar) or with (black bar) NEFA (1.2 mM, 12 h) in the absence (left bars) or presence (right bars) of NFκB inhibitor wogonin (100 μM). (G) Arithmetic means ± SEM (n = 9) of the CHOP/β-actin ratio in BRL-3A cells incubated without (white bar) or with (black bar) NEFA (1.2 mM, 12 h) in the absence (left bars) or presence (right bars) of NFκB inhibitor wogonin (100 μM). (H) Arithmetic means ± SEM (n = 9) of the NFκB p65/β-actin ratio in BRL-3A cells incubated without (white bar) or with (black bar) NEFA (1.2 mM, 12 h) in the absence (left bars) or presence (right bars) of NFκB inhibitor wogonin (100 μM). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 indicate significant difference from control; #p < 0.05, ##p < 0.01 indicate significant difference from NEFAs alone (one-way ANOVA).