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. 2019 Oct 2;7:202. doi: 10.3389/fcell.2019.00202

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Copyright © 2019 Zhang, Li, Zou, Guo, Zhang, Xia, Zhang, Yang and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of Orai1 silencing on ER stress protein levels in BRL-3A cells. (A) Original Western blots showing the protein expression of PERK, IRE1, ATF6, CHOP, GRP78, ORAI1, NFκB p65, and β-actin in BRL-3A cells incubated without or with NEFA (1.2 mM, 12 h) in the absence or presence of siOrai1. (B) Arithmetic means ± SEM (n = 9) of the PERK/β-actin ratio in BRL-3A cells incubated without (white bar) or with (black bar) NEFA (1.2 mM, 12 h) in the absence (left bars) or presence (right bars) of siOrai1. (C) Arithmetic means ± SEM (n = 9) of the IRE1/β-actin ratio in BRL-3A cells incubated without (white bar) or with (black bar) NEFA (1.2 mM, 12 h) in the absence (left bars) or presence (right bars) of siOrai1. (D) Arithmetic means ± SEM (n = 9) of the ATF6/β-actin ratio in BRL-3A cells incubated without (white bar) or with (black bar) NEFA (1.2 mM, 12 h) in the absence (left bars) or presence (right bars) of siOrai1. (E) Arithmetic means ± SEM (n = 9) of the GRP78/β-actin ratio in BRL-3A cells incubated without (white bar) or with (black bar) NEFA (1.2 mM, 12 h) in the absence (left bars) or presence (right bars) of siOrai1. (F) Arithmetic means ± SEM (n = 9) of the ORAI1/β-actin ratio in BRL-3A cells incubated without (white bar) or with (black bar) NEFA (1.2 mM, 12 h) in the absence (left bars) or presence (right bars) of siOrai1. (G) Arithmetic means ± SEM (n = 9) of the CHOP/β-actin ratio in BRL-3A cells incubated without (white bar) or with (black bar) NEFA (1.2 mM, 12 h) in the absence (left bars) or presence (right bars) of siOrai1. (H) Arithmetic means ± SEM (n = 9) of the NFκB p65/β-actin ratio in BRL-3A cells incubated without (white bar) or with (black bar) NEFA (1.2 mM, 12 h) in the absence (left bars) or presence (right bars) of siOrai1. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 indicate significant difference from control; #p < 0.05, ##p < 0.01, ###p < 0.001 indicate significant difference from NEFAs alone (one-way ANOVA).