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A–I
Spatial learning and memory were tested on the Barnes maze. Quadrant 1 (Q1) contains the target hole (red; A). Unilateral injection of AAV‐pDyn (B) or AAV‐eGFP (C) into naïve young adult mice (12 weeks age) did not influence the performance as compared to naïve controls (D) when tested 4 weeks after AAV injection. Mice treated 2 weeks after KA with AAV‐pDyn (E, H) performed equally to age‐matched naïve controls (D, G) 2 weeks (E) and 5.5 months (H) after treatment. By contrast, animals treated 2 weeks after KA with AAV‐ΔGFP (F, I) gradually lost this ability. Two‐way ANOVA revealed significance between AAV‐ΔGFP‐ and AAV‐pDyn‐treated groups for interaction 2 weeks (P = 0.0349) and 5.5 months (P = 0.0311) after AAV, respectively, at each time interval and quadrant (P < 0.0001) 2 weeks after AAV. Comparison of AAV‐pDyn injected with naïve animals revealed no differences.
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J–O
Epileptic mice, which were not able to learn the Barnes maze task 1 month after KA (J, M), AAV‐pDyn application restored spatial memory 1 (K) and 2 months (L) after treatment. Treatment with AAV‐ΔGFP did not result in improved memory (N, O). Two‐way ANOVA revealed significance for interaction (P = 0.0049) and quadrant (< 0.0001) comparing AAV‐ΔGFP with AAV‐pDyn‐treated animals at the later time interval.
Data information: Data represent mean ± standard deviation. Animal numbers: (B) and (C)
n = 9; (D) through (H)
n = 8; (I)
n = 5, note: Three mice had to be killed due to accelerating seizure activity and resulting weight loss; (J) through (O)
n = 7. ***
P < 0.001; **
P < 0.01; *
P < 0.05 by one‐way ANOVA and Dunnett post hoc test.
