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A–F
Double‐immunofluorescence labeling is depicted for pDyn and NeuN (A–C) or GFAP (D–F) in the ipsilateral dentate gyrus of KA‐treated and AAV‐pDyn‐injected mice. Enlarged view in (F) represents 15 × 30 μm.
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G
Mature Dyn B content (measured by a Dyn B‐specific EIA) in the dorsal hippocampus of mice treated with KA (blue symbols) or KA and AAV‐pDyn (red symbols) 1.5 (open symbols; n = 6) and 6 (filled symbols; n = 3) months after vector treatment. Naïve animals were age‐matched to the 1.5 months after AAV group. iH stands for ipsilateral hippocampus and cH for contralateral hippocampus. *P < 0.05; paired t‐test was used for comparison of ipsi‐ and contralateral hippocampi. Two‐way ANOVA was used to compare Dyn levels between the early and late time interval.
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H
Mature Dyn B content in the CSF of mice treated with KA (blue symbols) or KA and AAV‐pDyn (red symbols) 1.5 (open symbols; n = 6) and 7 (filled symbols; n = 4) months after vector treatment. **P < 0.01; one‐way ANOVA with Dunnett post hoc test
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I
The release of fully processed, mature Dyn B under different stimulation conditions was analyzed in microdialysates collected from the hippocampus of pDyn‐deficient (KO) animals 2 weeks after injection of AAV‐pDyn. Three baseline samples (BL; 25 min each) were collected. This was followed by 25 min low‐frequency stimulation (LF), 25 min baseline, and 25 min high‐frequency stimulation (HF; I).
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J
The microdialysis probe (red cross) was placed in the dentate gyrus, and the stimulation electrode (black cross) in the entorhinal cortex.
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K
Dyn B was quantified by EIA in the dialysate collected during different stimulation intensities. The red line represents the detection limit of the EIA. *P < 0.05; n = 4; one‐way ANOVA with Tukey post hoc test.
Data information: Data represent mean ± standard error of the mean.
