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. 2019 Sep 2;11(10):e9930. doi: 10.15252/emmm.201809930

Figure EV4. In vivo limiting dilution assay and metastasis formation assay results.

Figure EV4

  • A
    Effect of JQ1 and salinomycin treatment on the tumor growth of CRCM404 (n = 10). The gray area corresponds to the treatment period. Data represent mean ± SD.
  • B–D
    Reimplantation assay. Two‐week treated PDXs were reimplanted, in serial dilutions, into new recipient mice, and tumor growth was monitored (CRCM404). Each curve represents the growth kinetic from one individual injection.
  • E
    Effect of JQ1 and salinomycin treatment on the tumor growth of CRCM 494 (n = 10). The gray area corresponds to the treatment period. Data represent mean ± SD.
  • F–H
    Reimplantation assay. Two‐week treated PDXs were reimplanted, in serial dilutions, into new recipient mice, and tumor growth was monitored (CRCM494). Each curve represents the growth kinetic from one individual injection.
  • I
    Table showing the number of outgrowths generated in NSG mouse fat pads as a function of the amount of injected cells isolated from CRCM404, CRCM434, and CRCM494 PDXs following JQ1, salinomycin, JQ1/salinomycin combination, or placebo treatment.
  • J
    Effect of JQ1 and salinomycin treatments on the metastasis formation of CRCM404. Metastasis formation was monitored using bioluminescence imaging in mice livers and lungs.