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. 2019 Sep 6;39(9):BSR20190362. doi: 10.1042/BSR20190362

Figure 3. The Akt/mTOR signal pathway is not required for PD-L1 expression after cisplatin treatment.

Figure 3

(A,B) T24 and 5637 BC-derived cell lines were treated with different concentrations of cisplatin for 24 h (6.25, 12.5, or 25 μM, respectively), total protein was extracted and phosphorylation of Akt and mTOR was detected by Western blot. Total Akt and mTOR were used as the internal controls. (C,D) T24 and 5637 BC-derived cell lines were initially treated with 3 μM of Akti for 30 min, then with cisplatin (25 μM) for 24 h. Total protein was extracted and subjected to Western blot and qPCR assessments of Akt activation and levels of PD-L1. β-Actin was used as the internal control. Results are expressed as the mean ± S.D of triplicate samples. *P<0.05 compared with the control group.