Figure 4.

Illustration of the admissible window parameter. The admissible window defines the distance tolerance from the simulated imaging plane for RyR cluster centers to be considered ground true positives. (A) Statistical classifier CaCLEAN results as a function of the admissible window for low cluster density, no mitochondrial barriers (case 3). Twenty two images were simulated with equidistant spacing through the model volume. Solid lines represent mean values and shaded regions indicate one standard deviation of values. Figure S1 shows similar results across the four model permutations. In (B–D) an example image slice is shown, with clusters considered TP (ground truth) for admissible windows of (B) 300 nm, (C) 640 nm, and (D) 1,000 nm in magenta. An oblique view is shown above, along with FCa model results at t = 15 ms. Below, an axial view perpendicular to the imaging plane is shown (looking through the modeled volume, with clusters outside the admissible window shown in white). The admissible window is indicated in pink shading.