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. 2019 Oct 2;10:1214. doi: 10.3389/fpls.2019.01214

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Copyright © 2019 Furuya, Kimori and Tsukaya

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cultured plant samples and its processing images. (A–C) Gemmalings (12 days old) of the wild-type line, Tak-1 (A), the Mpan knockout line Mpan-1^ko (B), and the complementation line, pMpAN:MpAN-1/Mpan-1^ko (C). Bars, 3 mm. (D, E) Transverse views of a pre-processed micro-computed tomography (micro-CT) image (D) and the extracted plant image via manual image processing as shown in Figure 1 (E). e plastic eraser, p plant, t plastic tube. (F–H) Reconstructed three-dimensional (3D) images of Tak-1 (F), Mpan-1^ko (G), and pMpAN:MpAN-1/Mpan-1^ko (H). (I, J) Convex hull image of reconstructed images of Tak-1 (I) and Mpan-1^ko (J). The convex hull image and extracted plant image are shown in magenta and green, respectively.