Figure 6.

Expression levels of RNA-silencing genes in SMK plants infected with G5H. Expression levels of AGO genes (A), and DCL and RDR genes (B) in SMK soybean cultivar (rsv3-null) in response to G5H infection, ABA treatment, or both. Plants were collected 5 dpi for RNA extraction and RT-qPCR. Data are means ± standard deviation from three biological replicates. Statistical analysis was carried out for each gene using Student t-test; the means for plants treated with ABA alone, G5H alone, or the combination of ABA and G5H were individually compared with the mean for plants that were not treated with ABA or G5H: * and * indicate a significant increase or decrease, respectively, at P < 0.05. Additional analyses were carried for ABA-G5H in comparison with Mock-G5H where ns and * indicate non-significance and significance at P < 0.05, respectively.