Figure 4.

Both amino and carboxyl terminal truncations affect pUL37–EGFP trafficking. RPE-1 cell monolayers in chamber slides were infected with wild-type and each UL37–EGFP mutant virus (excluding Δ250C) at an MOI of 10 PFU/cell and analyzed by confocal microscopy 12 h post-infection. Wild-type pUL37–EGFP trafficked to the Golgi complex, as evidenced by the juxtanuclear localization and previous data from Desai et al. [35]. (A) N-terminal truncations of greater than 40 amino acids resulted in the dispersal of pUL37–EGFP localization to a more perinuclear localization. This was also seen with the functional Δ481N mutation. (B) C-terminal truncations all abolish the juxtanuclear localization pattern and they display different cellular localization, as judged by the fluorescence observed. Scale bar = 25 µm.