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Deleterious pUL37 alanine substitution mutants accumulate GFP fluorescence at a juxtanuclear site. RPE-1 cells in chamber slides were infected with EGFP-tagged pUL37 mutants or wild-type at an MOI of 10 PFU/cell and analyzed by confocal microscopy at 12 h post-infection. The pUL37–EGFP alanine mutant polypeptides 657–59, 680–82, 704–06, and 739–41 are observed with intense fluorescent signals at a juxtanuclear site. Scale bar = 25 µm.
