Figure 3.

Rescue of recombinant RVFVs with mutations that affect the NSs filament formation. (A) Schematic representations of NSs mutations introduced into the reverse genetics system. (B) Plaque assay of rWT and mutant viruses on Vero cells. Plaques were stained with crystal violet at 7 days p.i. (C) Western blot analysis of infected cells. Huh7 cells were infected with rWT and mutant viruses at an MOI of 1, and cell lysates were harvested and analyzed at 24 h p.i. with indicated antibodies. (D) Replication kinetics of WT, rWT and mutant viruses in Huh7 cells. Huh7 cells were infected with respective viruses with an MOI of 0.1 and the supernatants were collected at 0, 6, 12, 24, 36 and 48 h p.i. Viral titers were detected by plaque assay on Vero cells; error bars represent standard deviation.