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. 2019 Sep 4;11(9):820. doi: 10.3390/v11090820

Figure 3.

Figure 3

Production of recombinant adenovirus expressing GFP and B19NS1-GFP. (A) The schematic flowchart of recombinant Ad production. Both the Ad shuttle and proviral backbone plasmids are transfected into cells. After homologous recombination, a rAd proviral genome is generated, which further replicates to produce rAd virions as diagramed. (B) Recombinant adenovirus production. T-REx-293 cells were co-transfected using linearized pacAd5 CMVTetO2-GFP or pacAd5 CMVTetO2-B19NS1-GFP with linearized pacAd5F11P 9.2-100. After one week, GFP expression was monitored under a Nikon Eclipse Ti-S inverted microscope at 10× magnification.