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. 2019 Sep 4;11(9):819. doi: 10.3390/v11090819

Figure 1.

Figure 1

Schematic representation of KOS gB1 (A) and gB1s (B) and western blot analysis using anti-gB1s and anti-pK pAbs (C). SS, signal sequence; MPR, membrane-proximal region; TM, trans-membrane region; ectodomain and (cyto) cytodomain regions. Schematic representation of (A) full-length gB1 and (B) gB1s secreted protein obtained from the gB1 gene encoding the MPR and the TM regions, after having deleted 639 nucleotides and reconstructed the gene with the ectodomain and COOH cytodomain. The sequence from 872 to 904 represents the terminal sequence of the cytodomain present in gB1s. Sequence 68 to 76 is the lysine-rich region (pK) that binds HSPG. (C) WB analysis of gB1s recombinant protein (≈90 kD) using the anti-gB1s and anti-pK pAb (lane 3 and 7) and, as controls, full length KOS wt gB1 (120 kDa) (lane 1 and 5) or gBpK− mutant (lane 2 and 6) using the anti-gB1s and anti-pK pAbs respectively and mock uninfected cells tested with anti-gB1s and anti-pK pAbs (lanes 4 and 8). The anti-pK pAb is not able to recognize the KOS gBpK− mutant.