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Abundance of cytosolic AtHSP90 mRNA in WT and Atpao3 mutant and HSP90 protein levels. Leaves were treated with HS and HS + Rad for 1 h at 42 °C. (A) Reverse transcriptase (RT)-PCR of the four AtHSP90 cytosolic genes (AtHSP90 1–4) and relative intensities of bands normalized against ubiquitin. (B) Quantification of the HSP90 immunoreactive protein in control and HS- treated WT leaves, as well as in controls, HS and HS + Rad treated Atpao3 leaves.
