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. 2019 Aug 30;8(9):313. doi: 10.3390/plants8090313

Figure 3.

Figure 3

SmKAN expression in Selaginella moellendorffii by in situ hybridization. (a–c) SmKAN1 expression patterns. (a) SmKAN1 expression is detected in the vasculature in a transverse section of a shoot. (b) SmKAN1 expression is detected in the emerging microphyll primordia but not the meristem of a longitudinal section through a shoot. (c) SmKAN1 expression is detected in the emerging sporangia primordia and is maintained throughout their development in a longitudinal section through a strobilus. (d–f) SmKAN2 expression patterns. (d) SmKAN2 expression is detected in the vasculature in a transverse section of shoot. (e) SmKAN2 expression is detected in the emerging microphyll primordia but not the meristem of a longitudinal section through a shoot. (f) SmKAN2 expression is detected in the emerging sporangia primordia and is maintained throughout their development in a longitudinal section through a strobilus. (g–j) SmKAN3 expression results. (g) Cross section of a shoot, SmKAN3 expression is not detected. (h) Expression of SmKAN3 is not detected in the leaf primordia. (i) SmKAN3 expression is not detected during early sporangia development but is detected late in sporocoyte proliferation. (j) SmKAN3 expression in a nearly mature sporangium prior to meiosis. Scale bars: 50 µm (a,b,e,g); 20 µm (c,d,f,h); 10 µm (i,j). Asterisk indicates apical meristem; black arrowheads indicate phloem; black arrows indicate sporangia; li = ligule; m = microphyll.