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. 2019 Aug 30;8(9):313. doi: 10.3390/plants8090313

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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EhyKAN expression in Equisetum hyemale by in situ hybridization. (a) EhyKAN1 expression is detected in the apical meristem and emerging leaf primordia of a branch in a transverse section of a shoot. (b) EhyKAN1 expression is detected throughout the leaf primordia (innermost whorl) and then becomes restricted to the abaxial side of each leaf whorl (outer two whorls shown here) in a transverse section through a shoot. (c) EhyKAN2 expression is detected in the apical meristem and emerging leaf primordia of a branch in a transverse section of a shoot. (d) EhyKAN2 becomes restricted to the abaxial side of the leaf whorl in transverse section through a shoot. (e) EhyKAN3 expression is detected in the apical meristem of a branch in a transverse section of a shoot. (f) EhyKAN3 becomes restricted to the abaxial side of the leaf whorl in transverse section through a shoot. Scale bars: 50 µm (a); 100 µm (b–f). Asterisk indicates apical meristem; lines with l indicates leaf whorl; vb = vascular bundle.