Figure 7.

Brevilin A decreases the lethality observed in IAV-infected mice. (A) BALB/c mice were infected intranasally with three 50% lethal doses (LD₅₀) of PR8 virus. Brevilin A (25 mg/kg or 10 mg/kg) was intraperitoneally (i.p.) administered to mice 1 h after virus infection, and then once every other day for 6 days beginning on the day of infection (n = 8). Oseltamivir phosphate (20 mg/kg) used as a positive control was administered by oral gavage every day (n = 7). Vehicle (10% Lipovenos containing 0.2% DMSO, 10% PEG300 and 2.5% glycerol, n = 8) was used as a negative control. (B) The body weight of mice from each group was monitored daily from day 0 to day 14. The data represent means ± SD. (C) The survival rates of the mice were calculated. Animals falling below the threshold of 75% of their initial body weight were humanely euthanized. The p-value is shown (Log-rank (Mantel–Cox) test).