Table 1.
Gene | 5′-Reverse Primer-3′ | 5′-Forward Primer-3′ | #LNA Probe |
---|---|---|---|
BACT 1 | TCCCTGGAGAAGAGCTACGA | AAGAGCGCCTCTGGACAC | 9 |
CYPA 2 | CCTGAAGCATACGGGTCCT | AAAGACCACATGTTTGCCATC | 48 |
CLD-1 3 | CACCACTTTGCAAGCAACC | TGGCCACAAAGATGGCTATT | 3 |
CLD-2 4 | CTCGCGCCAAAGACAGAG | ATGAAGATTCCACGCAACG | 77 |
OCLN 5 | AAAGAGCTCTCTCGACTGGATAAA | AGCAGCAGCCATGTACTCTTC | 42 |
IL-8 | TTCTTCTTTATCCCCAAACTGG | CCACATGTCCTCAAGGTAGGA | 41 |
TNF-α | TCAGGGATTCAGGGATGTGT | GAAGCCCCAGTTCCAATTC | 77 |
IL-10 | TGCCTCCCACTTTCTCTTGT | TTCCTATGAGTGTAAGCGACTTTG | 23 |
1 β-actin, 2 cyclophilin A, 3 claudin-1, 4 claudin-2, 5 occludin.