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UPLC-MS/MS chromatograms of the analysis of zearalenone (ZEN) and its major metabolites zearalanone (ZAN), α-zearalenol (α-ZEL), β-zearalenol- (β-ZEL), α-zearalanol (α-ZAL), and β-zearalanol (β-ZAL) in a rumen fluid–buffer mixture that was not spiked with mycotoxins. The rumen fluid was taken from a cow before morning feeding and showed traces of ZEN (<LOQ). For each analyte, only the transition of the precursor ion to the product ion with the highest intensity (quantifier) is shown.
