Table 2.
Overview of the UPLC gradient programs used for the mycotoxin ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) methods in ESI+ and ESI− mode.
|
Analyte(s)
ESI+ |
Time
(min) |
MP A:B Ratio (v:v) |
| DON, 13C15-DON, DOM-1, NIV, ENN B, 15N3-ENN B, MPA, 13C17-MPA, ROQ-C, 13C22-ROQ-C |
0.00–0.50 0.50–5.50 5.50–7.50 7.50–7.70 7.70–10.0 |
95:5 Linear to 5:95 5:95 Linear to 95:5 95:5 |
|
Analyte(s)
ESI− |
Time
(min) |
MP C:D Ratio (v:v) |
| ZEN, 13C18-ZEN, ZAN, α-ZEL, β-ZEL, α-ZAL, β-ZAL | 0.00–0.50 0.50–3.50 3.50–4.90 4.90–5.00 5.00–7.00 |
70:30 Linear to 30:70 30:70 Linear to 70:30 70:30 |
Note: ESI+: positive electrospray ionization mode; ESI-: negative ESI; MP: mobile phase. MP A = 0.01% acetic acid (v/v) in H2O; MP B = 0.01% acetic acid (v/v) in MeOH; MP C = H2O; MP D = ACN. DON = deoxynivalenol, DOM-1 = deepoxy-deoxynivalenol, NIV = nivalenol, ENN B = enniatin B, MPA = mycophenolic acid, ROQ-C = roquefortine C, ZEN = zearalenone, ZAN = zearalanone, α-ZEL = α-zearalenol, β-ZEL = β-zearalenol, α-ZAL = α-zearalanol and β-ZAL = β-zearalanol.