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Isothermal titration calorimetry-binding profiles of (A) A179L as well as (B) A179L V73Y/G89Y interaction with Beclin BH3 peptide. Raw thermogram and a binding isotherm fitted with a one-site binding model are shown. All experiments were performed in triplicate. Data for wildtype A179L are from [44]. KD: Dissociation constant; ±: Standard deviation; NB: No binding.
