Table 4.
Brief comparison of autologous CAR-T constructs.
| Property | CAR-T Constructs | |||
|---|---|---|---|---|
| Kymriah® (Tisagenlecleucel-T; CTL019) | Yescarta® (Axicabtagene Ciloleucel; KTE-C19) | Lisocaptagene Maraleucel (Liso-cel, JCAR-017) | bb2121 | |
| Sponsor | Novartis | Gilead (Kite) | Celgene | Celgene/bluebird |
| KEGG Number # | D11386 | D11144 | Na | Na |
| Clinical stage | Approved by USFDA | Approved by USFDA | Phase III (NCT03575351) | Phase III (NCT03651128) |
| Base cost (US) | $475,000 for B-ALL; $373,000 for R/R DLBCL | $373,000 | Na | Na |
| Indication | B-ALL, R/R DLBCL | R/R DLBCL; PMBCL | R/R DLBCL; CLL | MM |
| T-cell source | Patient PBMCs; autologous; unspecified | Patient PBMCs; autologous; unspecified | Patient CD4 and CD8 T cells 1:1 ratio; autologous | Patient PBMCs; autologous |
| Vector | Lentivirus | Retrovirus | Lentivirus | Lentivirus |
| Antibody | Anti-CD19 mouse scFv FMC63 | Anti-CD19 mouse scFv FMC63 | Anti-CD19 mouse scFv FMC63 | Anti-BCMA |
| Costimulatory domain | 4-1BB | CD28 | 4-1BB | 4-1BB |
| Signaling domain | CD3ε | CD3ε | CD3ε | CD3ε |
| Hinge and transmembrane | CD8α | IgG1 Fc | IgG4 Fc spacer; CD28tm | CD8α |
| Other Markers | Nk | nk | EGFRt | nk |
| Ex vivo activation | CD3, CD28 | CD3, IL-2 | nk | CD3, CD28 |
| Lymphodepletion | Yes | yes | yes | yes |
| Time from leukapheresis to infusion | 21–28 days | 17 days | nk | 10 days |
| Dose | 0.2 to 5 × 106 CAR-positive viable T cells/kg | 0.4–2 × 106 anti-CD19 CAR-positive viable T cells/kg | 5 × 107 CD8+ and 5 × 107 CD4+ CAR-positive (not weight based) | 50–800 × 106 CAR-positive T cells (not weight based) |
Abbreviations: B-ALL, B-cell acute lymphoblastic leukemia; BCMA, B-cell maturation antigen; DLBCL, diffuse large B-cell lymphoma; CAR, chimeric antigen receptor; CD, cluster of differentiation; CLL, chronic lymphocytic leukemia; MM, multiple myeloma; Na, not applicable; nk, not known to these authors; PBMC, peripheral blood mononuclear cells; PMBCL, primary mediastinal large B-cell lymphoma; R/R, relapsed or refractory. #: Number. References used to build this table: [278,279,280,281,282,290,291,292,293,294,295].