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. 2019 Aug 29;11(9):802. doi: 10.3390/v11090802

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cannabis lettuce chlorosis virus (LCV) isolate transmitted via shoots.(a) Stunted growth of cannabis plant propagated from LCV infected shoots (left) compared to the growth of cannabis plant propagated from un-infected shoots (right). (b) Chlorotic leaves of the LCV infected cannabis plant shown in (a). (c) Un-infected cannabis leaves. (d) RT-PCR detection of LCV-Can infected (lanes 1–5) and un-infected (lanes 6–10) cannabis leaves using the primers RNA2-F-6090 and RNA2-F-7094 (Table 3). (e) Symptoms of propagated LCV-can infected plants in an authorized farm (AF). This severe stage was observed one-month post-fertilizer arrest, traditionally done before flower pickup. (f) A symptomatic flower of LCV infected propagated cannabis plant dissected to four samples (1–4). (g) RT-PCR of LCV infected cannabis-dissected flower (lanes 1–4) using the primers RNA2-F-7628 and RNA2-F-8189 for RNA2 (Table 3). MW-Molecular weight, M-Marker, (-) Negative control.