Table 1.
Next generation sequencing results of constructed libraries.
| Small RNA | Total RNA | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Reference Genome a | Complete Genome (nt) | No. Contigs b | Coverage c (%) | Depth d | Identity e | No. Contigs | Coverage (%) | Depth | Identity |
| Lettuce chlorosis virus (LCV) RNA1 | 8591 | 22 | 1054 (12.3%) | 5.7 | 97.25 | 39 | 7103 (82.7%) | 2671.7 | 89.78 |
| Lettuce chlorosis virus (LCV) RNA2 | 8556 | 38 | 2091 (24.4%) | 5.3 | 97.32 | 52 | 6722 (78.6%) | 2499.3 | 92.61 |
a GenBank reference genome of LCV (accession Nos. of RNA1, FJ380118 and RNA2, FJ380119), with which the assembled contigs had high similarity. b No. of contigs aligned to the reference genome. c Percentage of LCV reference genome covered by the assembled contigs. d The average of the No. of times each nucleotide of the reference genome was covered by the identified sequences. e The average (%) of nucleotide sequence identity of all contigs with the GenBank reference genome sequence.