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. 2019 Sep 13;11(9):533. doi: 10.3390/toxins11090533

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Extraction and purification scheme for CTX1B. Fish flesh was spiked with Food and Drug Administration (FDA) guidance level (0.01 ppb) of pure CTX1B and after extraction/purification, the sample and the tenfold dilution were analyzed by fluorescent sandwich ELISA. (B) Extraction and purification scheme for the evaluation of matrix effects at each step of the extraction/purification procedure. 0.01 ppb of pure CTX1B was spiked into fish flesh and after extraction/purification each extract (Samples B-1~B-4) was analyzed by fluorescent sandwich ELISA. (C) Extraction and purification scheme for direct evaluation of the matrix effects of each extract. 0.01 ppb of pure CTX1B was added to each extract obtained by the extraction/purification procedure (Samples C-1~C-4) and each sample was analyzed by fluorescent sandwich ELISA.