Table 1.
mAb | Kd (nM) | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
15 | 16 | 17 | 18 | CTX1B | CTX3C | 51-hydroxy-CTX3C | BTX-A | BTX-B | OA | MTX | |
10C9 | 0.8 | NI | ND | ND | ND | 2.8 | ND | NI | NI | NI | NI |
3D11 | NI | 8.6 | ND | ND | ND | 122 | ND | 43000 | NI | NI | NI |
8H4 | ND | ND | 48 | ND | 20.4 | 3200 | 13.6 | NI | NI | NI | NI |
3G8 | ND | ND | ND | 1.5 | 15 | NI | ND | NI | NI | NI | ND |
1 The Kd’s were determined by competitive ELISA. ND: not determined. NI: no inhibition was observed at the maximum concentration of each inhibitor (for 10C9, 250 μM 16, 100 μM BTX-A, 100 μM BTX-B, 100 μM OA, 25 μM MTX; for 3D11, 250 μM 15, 100 μM BTX-A, 100 μM BTX-B, 100 μM OA, 25 μM MTX; for 8H4, 100 μM BTX-A, 100 μM BTX-B, 100 μM OA, 25 μM MTX; for 3G8, 1.9 μM CTX3C, 10 μM BTX-A, 100 μM BTX-B, 100 μM OA).