Figure 2.

Neutralization of G-CSFR does not influence C. perfringens α-toxin-induced myonecrosis. Mice were intramuscularly injected with 1 × 10⁷ CFU of C. perfringens Strain 13 (Wild-type Cp-infected) or TGY medium as a control (Control). To neutralize G-CSFR, a specific antibody against mouse G-CSFR (Anti-G-CSFR) or an isotype control antibody (Isotype) was intraperitoneally administered to the C. perfringens-infected mice shortly after the injection of C. perfringens. (A) Representative H&E-stained sections of three independent experiments are shown. (B) The diameters of at least 100 muscle fibers of three independent experiments were measured. (C) Plasma creatine kinase activities were determined using a creatine kinase activity assay kit (n = 11 per condition). One-way ANOVA or the two-tailed Student’s t-test was employed to assess significance. Values are the mean ± standard error. *** p < 0.001; n.s., not significant.