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. 2019 Aug 30;11(9):803. doi: 10.3390/v11090803

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Maximum-likelihood tree based on replication-associated protein (rep) amino acid sequence (~306 aa) of 28 smacovirus sequences. The tree is midpoint rooted and was built in RAxML v8.2 software using Le and Gascuel (LG) substitution-rate matrix with gamma distribution (+G), invariant sites (+I), and empirical amino acid frequency (+F) in accordance to ProtTest analysis. Bootstrap was performed with 1,000 replicates and values equal to 70 or more are represented by asterisks. Nodes in grey area belongs to Porprismacovirus genus. GenBank accession number of the proposed Avian associated porprismacovirus identified in this study is shown. Details about the sequences of the phylogenetic tree are shown in Table S3.