Table 3.
VH, Vκ, and Vλ scaffolds used as foundations for the synthetic and semisynthetic libraries. We did not include HuCAL PLATINUM in the table since the scaffolds are not natural germline genes but consensus sequences.
| Ylanthia | pIX V3.0 | PHILODiamond | ALTHEA Gold Libraries | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| VH | Kappa | Lambda | VH | Kappa | Lambda | VH | Kappa | Lambda | VH | Kappa | Lambda |
| 1–18 | 1–6 | 1–40 | 1–69 | 1–39 | N/A | 3–23 | 3–20 | 3–19 | 3–23 | 3–20 | N/A |
| 1–46 | 1–9 | 1–47 | 3–23 | 3–11 | 4–1 | ||||||
| 1–69 | 1–12 | 1–51 | 5–51 | 3–20 | |||||||
| 3–7 | 1–15 | 2–11 | 4–1 | ||||||||
| 3–11 | 1–27 | 2–23 | |||||||||
| 3–15 | 1–39 | 3–1 | |||||||||
| 3–21 | 3–15 | ||||||||||
| 3–23 | 3–20 | ||||||||||
| 3–53 | |||||||||||
| 3–74 | |||||||||||
| 5–51 | |||||||||||
| 6–1 | |||||||||||
| HCDR3 (loop lengths) | |||||||||||
| 4–25 | 3–15 | 4–7 | Natural (1–25) | ||||||||
Color code: Orange: VH scaffolds paired with both Vκ, and Vλ. Yellow: IGHV3-23 is paired with both Vκ, and Vλ and used in all the libraries. Dark green: scaffolds used in all the libraries. Light green: scaffolds used in more than one library. N/A: non-applicable; these are kappa only libraries.