Figure 5.

Co-expression of isolated PSIs and cardosin constructs with the dominant negative mutant of RabF2b. (A) Schematic representation of the constructs used in this assay. (B) Co-expression of SP::PSI-A::mCh (c–d) and SP::PSI-B::mCh (e–f) with the dominant negative mutant RabF2b^S24N. SP::mCh::C-terA (a–b) was used as a positive control for the efficiency of the mutant. Note the accumulation in the cell wall in the positive control and SP::PSI-B::mCh, but not for SP::PSI-A::mCh. (C) Co-expression of RabF2b^S24N with cardosin fusion proteins: (a–b) Cardosin A∆Cter, (c–d) Cardosin B∆Cter, (e–f) Cardosin A::PSIB∆Cter, and (g–h) Cardosin B::PSIA∆Cter. All the proteins are insensitive to the blockage of this specific pathway. All observations and images were acquired 3 days post-infiltration. Images were analyzed and processed using ImageJ/Fiji software. Scale bars: 50 µm.