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. 2019 Sep 17;11(9):869. doi: 10.3390/v11090869

Figure 3.

Figure 3

The expression of the D-fused pig PR39 [36], or human LL37 [32,33,34,35] cathelicidins killed/prevented the growth of E. coli cells expressing the D-fusions. Single colonies of cells with plasmid p613 (pcIpR-Dcoe-timm), p625 (pcIpR-Dcoe-PR39-timm, where D was fused to a 42 AA pig cathelicidin), and p627 (pcIpR-Dcoe-LL37-timm, where gpD was fused to the 39 AA human cathelicidin) were inoculated into LB-Amp50 medium (Methods and grown at 30 °C in shaking water bath to stationary phase. A 1-mL aliquot was removed and serially diluted into buffer (Methods), and 5-uL aliquots of the dilutions were spotted in quintuplicate onto LB-Amp50 agar plates, one heated to 30 °C and the other to 42 °C, and then moved to the respective incubators for the spots to dry with lids ajar, then inverted with continued incubation overnight. Titers were determined by averaging the cfu (visualized by dissecting microscope) per five spots X dilution factor X 200.