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. 2019 Sep 6;11(9):517. doi: 10.3390/toxins11090517

Figure 2.

Figure 2

Reverse-phase high-performance liquid chromatography (RP-HPLC) of the hemocyte samples eluted at 40% acetonitrile (ACN). After the fractionation step using a Sep-Pak® C18 column, the samples eluted in 40% ACN were subjected to a Jupiter® C18 semi-preparative column, with a linear gradient from 2% to 60% ACN (dotted line) in acidified water for 60 min. The fractions were collected manually and submitted to a test of the inhibition of microbial growth in a liquid medium. Peaks with squares and circles indicate antimicrobial activities against the tested microorganisms: Escherichia coli SBS 363 (●), Microccocus luteus A270 (○), Candida albicans MDM 8 (■), and Aspergillus niger (□) (A). Serrulin, indicated with an arrow, was submitted to RP-HPLC using a Jupiter® C18 analytic-column, with a linear gradient from 13% to 43% ACN (dotted line) in 0.05% TFA, for 60 min. The fractions were collected manually and submitted to a test of the inhibition of microbial growth in a liquid medium and mass analyses (B).