Figure 1.

Molecular characteristics of GuMPV B29. The genome organization of the novel virus is depicted at the top. Identified open reading frames (ORFs) are illustrated by colored rectangles: Nonstructural proteins are in green (N: nucleoprotein; P: phosphoprotein, L: polymerase), envelope glycoproteins are in yellow (F: fusion protein; SH: small hydrophobic protein; G: glycoprotein), and the matrix protein (M) is in pink. The scale bar on top represents genomic position in Kb. The phylogenetic placement of GuMPV B29 within the genus Metapneumovirus based on the complete amino acid sequence of the L protein is shown at the bottom. The two species Human metapneumovirus (AMPV) and Avian metapneumovirus (AMPV) are indicated on the right, and the murine pneumovirus (MPV) was used as an outgroup. Accession numbers are indicated in parentheses. Officially assigned viral sub-groups are indicated by letters (A1-2, B1-2 for HMPV, A–D for AMPV), while recently identified viruses that lack official taxonomic designations are indicated by circles with the virus identified in this study indicated by the filled green circle. The tree was built with the maximum likelihood method [47] using MEGA 7 [43] based on the Le Gascuel model [49], identified as the best-fitting model by the model test in MEGA. A discrete Gamma distribution was used to model evolutionary rate differences among sites, branch lengths are proportional to genetic distances as indicated by the scale bar, and the outcome of the bootstrap analysis [48] is shown next to the nodes.