Table 4.
List of primer and probe sequences for RT-qPCR.
| Gene | Pair of Primers (FWD and REV) | Probe |
|---|---|---|
| ACTB | GGATCAGCAAGCAGGAGTATG; | AGAAAGGGTGTAACGCAACTAA |
| TCGTCCACCGCAAATGCTTCTAGG | ||
| HIF1a | GTCTGCAACATGGAAGGTATTG; | GCAGGTCATAGGTGGTTTCT |
| ACTGCACAGGCCACATTCACGTAT | ||
| HK2 | GCAGAAGGTTGACCAGTATCTC; | CCAAGCCCTTTCTCCATCTC |
| CACATGCGCCTCTCTGATGAGACC | ||
| PFKM | GCATCCCATTTGTGGTCATTC; | GTCACAGGTTGTGCAGATAGT |
| AATGTCCCTGGCTCAGACTTCAGC | ||
| PKM2 | CTGTGGCTGGACTACAAGAA; | CTGCTTCACCTGGAGAGAAATA |
| AAGTGGGCAGCAAGATCTACGTGG | ||
| SLC2A1 | CTGGGCAAGTCCTTTGAGAT; | GTGACACTTCACCCACATACA |
| AGTACACACCGATGATGAAGCGGC | ||
| SLC2A3 | AGGATGCAGGTGTTCAAGAG; | GCCCTTTCCACCAGAAATAGA |
| CGGCGCGGGTGTGGTTAATACTAT | ||
| LDHA | AGATTCCAGTGTGCCTGTATG; | ACCTCTTTCCACTGTTCCTTATC |
| AGTGGAATGAATGTTGCTGGTGTCTCT | ||
| PGC-1a | AGAGCGCCGTGTGATTTAT; | CTCCATCATCCCGCAGATTTA |
| ACCTGACACAACACGGACAGAACT | ||
| NRF1 | GTATCTCACCCTCCAAACCTAAC; | CCAGGATCATGCTCTTGTACTT |
| TGCAGCACCTTTGGAGAATGTGGT | ||
| NRF2 | GCAATCTGCTACACCTACTACC; | TCCCAGGTGAGCTTCTATCT |
| AAGCAGCCAAAGTACAAAGAGCGC | ||
| TFAM | GTTGGAGGGAACTTCCTGATT; | CTGACTTGGAGTTAGCTGTTCT |
| AAGATGCTTATAGGGCGGAGTGGC | ||
| SOD1 | GTGCAGGGCATCATCAATTTC; | GGCCTTCAGTCAGTCCTTTAAT |
| AAGTAATGGACCAGTGAAGGTGTGGG | ||
| SOD2 | GCCTACGTGAACAACCTGAA; | GAAGAGCTATCTGGGCTGTAAC |
| TCACCGAGGAGAAGTACCAGGAGG | ||
| SOD3 | GCTGGCGCTACTGTGTT; | CGTGACCTTGGCGTACAT |
| AACTCTGACTCGGCGGAGTGGATC | ||
| CAT | CTGGAGCACAGCATCCAATA; | TCATTCAGCACGTTCACATAGA |
| ATTGGCAGTGTTGAATCTCCGCAC | ||
| GPX1 | CAGTCGGTGTATGCCTTCTC; | GCTCGTTCATCTGGGTGTAG |
| TATCGAGAATGTGGCGTCCCTCTGA | ||
| PRDX3 | CCTTTGTGTGTCCTACAGAAATTG; | TCCAGGCAAGATGGCTAAAG |
| TGTGAAGTTGTCGCAGTCTCAGTGG |