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. 2019 Sep 8;11(9):833. doi: 10.3390/v11090833

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Immunofluorescence assay (IFA) showing the binding of (a) GII.4 WT VLPs and its two mutants (b) D374A and (c) G443A VLPs to lettuce tissues as well as (d) control negative with no VLP added, Magnification: 40×. (B) Quantification of fluorescent intensity. Means with different letters differ significantly.