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. 2019 Sep 7;11(9):520. doi: 10.3390/toxins11090520

Figure 2.

Figure 2

Disruption of HDAC genes in A. niger FGSC A1279 and phenotypic plates. (A) Each HDAC coding sequence was replaced with the pyrG selection marker by double homologous recombination. Positive deletion strains were confirmed by PCR screening with three different primer pairs. (B) Morphological phenotypes of disruptants on a stress culture plate on day seven. (C) Morphological phenotypes of the indicated strain in various chemical substances treated cultures after five days of growth. (D) Wild-type strains and the ΔhosA, and ΔhdaA mutants in liquid CD medium on day four. Abbreviations of chemical substances are listed in Table 2.